This is supplementary material for the paper: "The role of DNA-binding specificity in the evolution of bacterial regulatory networks" A MS Word document with supplementary methods, tables and figures is available here. A MS Excel spreadsheet containing the data used for the figures in the paper is available here. The authors, Irma Lozada-Chávez, Vladimir Espinosa Angarica, Julio Collado-Vides and Bruno Contreras-Moreira